#Please insert up references in the next lines (line starts with keyword UP) UP arb.hlp UP glossary.hlp #Please insert subtopic references (line starts with keyword SUB) #SUB subtopic.hlp # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} #************* Title of helpfile !! and start of real helpfile ******** TITLE Design Sequencing Primers OCCURRENCE ARB_NT/Probes/Design Sequencing Primers DESCRIPTION This module will search for primers for all positions. The best result is one primer for all (marked) taxa, the worst case are n primers for n taxa. * does only search in marked sequences. * sequences need to be aligned. Searches for sections in alignment which require the lowest number of primer combinations. You have to specify the maximum allowed number of different primers. Specify '1' to report only primers valid for all marked species. The module outputs a list of primer candidates, probably outputs hundreds of pages. Checking these primers requires a lot of manual work. Press ctrl-C to abort the program. SECTION Field experience Bad sequences confuse the algorithm. Workaround: unmark them. NOTES None EXAMPLES None WARNINGS None BUGS No bugs known