#Please insert up references in the next lines (line starts with keyword UP) UP arb.hlp UP glossary.hlp UP pt_server.hlp #Please insert subtopic references (line starts with keyword SUB) SUB probe_param.hlp SUB probedesignresult.hlp # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} #************* Title of helpfile !! and start of real helpfile strunk ******** TITLE Probe Design OCCURRENCE ARB_NT/Probes/Design Probes DESCRIPTION Searches for potential probe target sites within the sequence entries of the selected 'PT_SERVER' database. First mark (a single or a group of) species (see LINK{mark.hlp}) for which probe target sites should be designed. Configure the parameters (see LINK{probe_param.hlp}) and press 'GO' to start the probe design. NOTES The 'PT_SERVER' database ('*.arb' and '*.arb.pt') stored in '$ARBHOME/lib/pts' is used for probe target searching (not the current database!). The 'PT_SERVER' database has to be up-to-date or result calculated by probe design will be inaccurate (see LINK{probe_param.hlp}). EXAMPLES None WARNINGS Take care that only and all species are marked for which a group specific probe has to be designed! If the displayed tree is used for species marking, consider that species belonging to the specificity group but not contained in this tree will remain unmarked and treated as non-targets. Consequently, useful target sites may be not detected. Similarly marked species not related to the target species and not contained in the displayed tree will be treated as targets. BUGS No bugs known