#Please insert up references in the next lines (line starts with keyword UP) UP arb.hlp UP glossary.hlp #Please insert subtopic references (line starts with keyword SUB) #SUB subtopic.hlp # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} #************* Title of helpfile !! and start of real helpfile ******** TITLE Column statistic OCCURRENCE ARB_NT/SAI/Create SAI from Sequences/Positional Variability ... DESCRIPTION Calculates the base and frequencies positional variability for each column independently. It uses the parsimony method to find the minimum number of mutations for each site. The result can be used by: - Parsimony to weight the characters properly - Neighbour joining to estimate the distances more accurately. - Filter @@@@ Result: '.' Less than 10% valid characters '0123456789ABCDE...' The higher the number the more conserved +2 half mutations eg .'7' half number of mutations than '5' NOTES Use the biggest tree you have. NOTE @@@ Compare it to consensus and max frequency. WARNINGS if you have only small trees (<100 species), forget this function. BUGS No bugs known