#       main topics:
UP      arb.hlp
UP      glossary.hlp
UP      probedesign.hlp

#       sub topics:
#SUB     subtopic.hlp

# format described in ../help.readme


TITLE           Probe design (Expert)

OCCURRENCE	ARB_NT/Probes/Design Probes/EXPERT

DESCRIPTION	See LINK{bonds.hlp} for upper area.

		Define threshold for splitting melting domains:

			Type a value to the 'Threshold for Splitting' subwindow.
			This value is subtracted from the mean of all pairing
			values of the potential probe target hybrid. If the
			resulting value is lower than that of a particular
			mispairing, it is assumed that the remaining base paired
			stretches melt independently. The dissociation
			temperature of the whole hybrid is that of the more
			stable part of it.

NOTES		None

EXAMPLES	None

WARNINGS	The criteria used for probe target site ranking are based on
		experimental experience. However, optimum conditions have to be
		determined experimentally for the particular probe target
		hybrids.

BUGS		No bugs known
