#Please insert up references in the next lines (line starts with keyword UP)
UP	arb.hlp
UP	glossary.hlp
UP	probedesign.hlp

#Please insert subtopic references  (line starts with keyword SUB)
#SUB	bonds.hlp

# Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain}

#************* Title of helpfile !! and start of real helpfile ********
TITLE           Probe design (Expert)

OCCURRENCE	ARB_NT/Probes/Design Probes/EXPERT

DESCRIPTION	See LINK{bonds.hlp} for upper area.

		Define threshold for splitting melting domains:

			Type a value to the 'Threshold for Splitting' subwindow.
			This value is subtracted from the mean of all pairing
			values of the potential probe target hybrid. If the
			resulting value is lower than that of a particular
			mispairing, it is assumed that the remaining base paired
			stretches melt independently. The dissociation
			temperature of the whole hybrid is that of the more
			stable part of it.

NOTES		None

EXAMPLES	None

WARNINGS	The criteria used for probe target site ranking are based on
		experimental experience. However, optimum conditions have to be
		determined experimentally for the particular probe target
		hybrids.

BUGS		No bugs known