#Please insert up references in the next lines (line starts with keyword UP) UP arb.hlp UP glossary.hlp #Please insert subtopic references (line starts with keyword SUB) SUB translate_dna_2_pro.hlp # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} #************* Title of helpfile !! and start of real helpfile ******** TITLE Realign DNA OCCURRENCE ARB_NT/Sequence/Align sequences/Realign nucleic acid according to aligned protein DESCRIPTION If you have translated DNA sequences into protein sequences and then have aligned the resulting protein sequences, this function can be used to re-align the DNA sequences according to the (modified) protein alignment. Simply select the protein and the corresponding DNA alignment and press 'REALIGN' to re-align the DNA sequences of all marked species. NOTES If the species contains a field 'transl_table' (which should contain the translation table number as used by the EMBL database) it is used to detect the correct translation table. If no such field is defined a translation-table-auto-detection takes place. If the species contains a field 'codon_start' it is overwritten with '1' to make further translations work properly. EXAMPLES None WARNINGS If you have single DNA bases at the beginning (or end) of the DNA sequence there have to be corresponding proteins in the protein sequence. Otherwise translation will fail. Normally, if you use codon start other than 1 during translation, a 'X' will be inserted into the resulting protein sequence. That X is required by the re-aligner to skip up to 3 DNA positions, while it is trying to find the correct ORF. Missing X's at protein sequence start are a very common cause, for various kinds of error messages from the re-aligner. BUGS No bugs known