arb_item:Clustalv Alignment (DNA) itemmethod:(tr '"%//' '>' clus_in; RUN_IN_WINDOW((clustalv /output=PIR /infile=clus_in /align /ktuple=$KTUP /window=$WIN $Trans /fixedgap=$FIXED /floatgap=$FLOAT;cat clus_in.pir |sed "s/^>DL;/#/g" |sed "s/^>P1;/%/g" |sed "s/^\*$//g" >out1; )); /bin/rm -f clus_in* in1) itemhelp:clustal_help arg:KTUP argtype:slider arglabel:K-tuple size for fast pairwise search argmin:1 argmax:10 argvalue:2 arg:WIN argtype:slider arglabel:Window size argmin:1 argmax:10 argvalue:4 arg:Trans argtype:chooser arglabel:Transitions weighted? argchoice:Yes:/TRANSIT argchoice:No: arg:FIXED argtype:slider arglabel:Fixed gap penalty argmin:1 argmax:100 argvalue:10 arg:FLOAT arglabel:Floating gap penalty argtype:slider argmin:1 argmax:100 argvalue:10 in:in1 informat:flat insave: out:out1 outformat:flat item:Clustalw DNA Alignment itemmethod:(tr '"%//' '>' clus_in;RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align -ktuple=$KTUP_ -window=$WIN_ -topdiags=$WIN_ $MATRIX_ -gapopen=$FIXED_ -gapext=$FLOAT_ -transweight=0.5 -type=DNA -DNAMATRIX=$MATRIX_;cat clus_in.pir |sed "s/^>DL;/#/g" |sed "s/^>P1;/%/g" |sed "s/^\*$//g" >out1; )); /bin/rm -f clus_in* in1) itemhelp:clustal.help arg:KTUP argtype:slider arglabel:K-tuple size for fast pairwise search argmin:1 argmax:10 argvalue:2 arg:WIN argtype:slider arglabel:Window size for fast pairwise search argmin:1 argmax:10 argvalue:4 arg:MATRIX_ argtype:chooser arglabel:Weighting matrix argchoice:BLOSUM:BLOSUM argchoice:PAM:PAM argchoice:GONNET:GONNET argchoice:ID:ID arg:FIXED_ argtype:slider arglabel:Gap Open Panelty argmin:1 argmax:100 argvalue:8.7 arg:FLOAT_ arglabel:Gap Extend Panelty argtype:slider argmin:.001 argmax:1 argvalue:.1 in:in1 informat:flat insave: out:out1 outformat:flat item:Clustalw Protein Alignment itemmethod:(tr '"%//' '>' clus_in;RUN_IN_WINDOW((clustalw -output=PIR -infile=clus_in -align -ktuple=$KTUP_ -window=$WIN_ -topdiags=$WIN_ $MATRIX_ -gapopen=$FIXED_ -gapext=$FLOAT_ -type=PROTEIN;cat clus_in.pir |sed "s/^>DL;/#/g" |sed "s/^>P1;/%/g" |sed "s/^\*$//g" >out1; ));\/bin/rm -f clus_in* in1) itemhelp:clustalw.help arg:KTUP_ argtype:slider arglabel:K-tuple size for fast pairwise search argmin:1 argmax:10 argvalue:2 arg:WIN_ argtype:slider arglabel:Window size and top diagonals for fast alignment argmin:1 argmax:10 argvalue:4 arg:MATRIX_ argtype:chooser arglabel:Weighting matrix argchoice:BLOSUM:BLOSUM argchoice:PAM:PAM argchoice:GONNET:GONNET argchoice:ID:ID arg:FIXED_ argtype:slider arglabel:Gap Open Panelty argmin:1 argmax:100 argvalue:8.7 arg:FLOAT_ arglabel:Gap Extend Panelty argtype:slider argmin:.001 argmax:1 argvalue:.1 in:in1 informat:flat insave: out:out1 outformat:flat item:Assemble Contigs itemmethod: (tr '"%//' '>' in1.tmp;CAP2 in1.tmp $OVERLAP $PMATCH > out1;/bin/rm -f in1.tmp) itemhelp:CAP2.help arg:OVERLAP argtype:slider arglabel:Minimum overlap? argmin:5 argmax:100 argvalue:20 arg:PMATCH argtype:slider arglabel:Percent match required within overlap argmin:25 argmax:100 argvalue:90 in:in1 informat:flat out:out1 outformat:gde