#Please insert up references in the next lines (line starts with keyword UP) UP arb.hlp UP glossary.hlp #Please insert subtopic references (line starts with keyword SUB) # Hypertext links in helptext can be added like this: LINK{ref.hlp|http://add|bla@domain} #************* Title of helpfile !! and start of real helpfile ******** TITLE How is the consensus calculated? OCCURRENCE ARB_NT/SAI/Create SAI using../Consensus ARB_EDIT4/Properties/Consensus definition DESCRIPTION What to do with gaps? If a column contains only gaps the result will be shown as '='. Define whether to use or to ignore gaps entirely: If you count gaps and the gap frequency exceeds 'threshold for gaps', the result will be shown as '-'. If the switch is 'off', the algorithm will virtually remove all gaps. That means if you have a column with two 'A's and 500 gaps the program thinks of 100% 'A'. (If the switch is 'on', the relative number of 'A's would be 2%) Simplify using IUPAC Define whether the most frequent base or the IUPAC code (more then one base) should be shown at the particular position by pressing the respective checkbox after the 'simplify' prompt. Characters are used for IUPAC encoding only if their frequency exceeds 'threshold for character'. Example: If you have 40% 'A', 10% 'C', 40% 'G' and 10% 'T' and 'threshold for character' is set to 20%, the program looks for a iupac code only for 'A' and 'G'. Note: The IUPAC codes are displayed after pressing the 'show IUPAC' button. Show as upper or lower case? Define whether the character is displayed in upper or lower case or whether a dot is displayed. Define upper and lower limit: If the percentage of a character is above or equal to the upper limit, the character is displayed in upper case. If the percentage of a character is above or equal to the lower limit and below the upper limit, the character is displayed in lower case. Otherwise a dot ('.') is displayed. NOTES None EXAMPLES None WARNINGS IUPAC symbols in source sequences are ignored. BUGS 'LINK{consensus.ps}' is broken for more than 10 years now. Seems that nobody ever took a look.